Interruption of blood flow to the brain sets off a cascade of biophysical and biochemical events that ultimately result in neuronal death. At Advanced Neural Biosciences, Inc., we have identified a number of protocols and compounds that can depress cerebral metabolism and improve blood flow to the brain after such ischemic events. These technologies also permit ice-free cryopreservation of the brain under circumstances where there is a delay between circulatory arrest and start of brain cryopreservation procedures.